GTF2A1 Antibody (PCRP-GTF2A1-1F2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13933
![Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]](https://resources.bio-techne.com/images/products/GTF2A1-Antibody-PCRP-GTF2A1-1F2-Immunocytochemistry-Immunofluorescence-NBP3-13933-img0002.jpg "Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # PCRP-GTF2A1-1F2
Concentration
0.2 mg/ml
Product Summary for GTF2A1 Antibody (PCRP-GTF2A1-1F2)
Immunogen
Recombinant full-length human GTF2A1 protein (Uniprot: P52655)
Reactivity Notes
Predicted to react in Mouse and Rat.
Localization
Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
41.51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14054)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for GTF2A1 Antibody (PCRP-GTF2A1-1F2)
Immunocytochemistry/ Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]
Immunocytochemistry/Immunofluorescence: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using GTF2A1 Antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).![Flow Cytometry: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]](https://resources.bio-techne.com/images/products/GTF2A1-Antibody-PCRP-GTF2A1-1F2-Flow-Cytometry-NBP3-13933-img0001.jpg "Flow Cytometry: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]")
Flow Cytometry: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]
Flow Cytometry: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933] - Flow cytometric analysis of PFA-fixed HeLa cells. GTF2A1 antibody (PCRP-GTF2A1-1F2) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).![Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]](https://resources.bio-techne.com/images/products/GTF2A1-Antibody-PCRP-GTF2A1-1F2-Protein-Array-NBP3-13933-img0004.jpg "Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]")
Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933]
Protein Array: GTF2A1 Antibody (PCRP-GTF2A1-1F2) [NBP3-13933] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2A1 Antibody (PCRP-GTF2A1-1F2).Applications for GTF2A1 Antibody (PCRP-GTF2A1-1F2)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunoprecipitation
1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: GTF2A1
Alternate Names
General transcription factor IIA subunit 1, general transcription factor IIA, 1, 19/37kDa, glucose regulated protein, 58kD pseudogene, MGC129969, MGC129970, TF2A1, TFIIA, TFIIA alpha, p55, TFIIA-42, TFIIAL, transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit
Gene Symbol
GTF2A1
UniProt
Additional GTF2A1 Products
Product Documents for GTF2A1 Antibody (PCRP-GTF2A1-1F2)
Product Specific Notices for GTF2A1 Antibody (PCRP-GTF2A1-1F2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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