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GTF2A1L Antibody (3E4)

Novus Biologicals, part of Bio-Techne | Catalog # H00011036-M05

Novus Biologicals, part of Bio-Techne
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H00011036-M05

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ALF (NP_006863, 251 a.a. ~ 348 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQVSQTNSNVESVLSGSASMAQNLHDESLSTSPHGALHQHVTDIQLHILKNRMYGCDSVKQPRNIEEPSNIPVSEKDSNSQVDLSIRVTDDDIGEIIQ

Localization

Nuclear

Specificity

ALF - TFIIA-alpha/beta-like factor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for GTF2A1L Antibody (3E4)

Western Blot: GTF2A1L Antibody (3E4) [H00011036-M05]

Western Blot: GTF2A1L Antibody (3E4) [H00011036-M05]

Western Blot: GTF2A1L Antibody (3E4) [H00011036-M05] - Analysis of ALF expression in transfected 293T cell line by ALF monoclonal antibody (M05), clone 3E4. Lane 1: ALF transfected lysatE (52.4 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: GTF2A1L Antibody (3E4) [H00011036-M05]

Immunocytochemistry/ Immunofluorescence: GTF2A1L Antibody (3E4) [H00011036-M05]

Immunocytochemistry/Immunofluorescence: GTF2A1L Antibody (3E4) [H00011036-M05] - Analysis of monoclonal antibody to GTF2A1L on HeLa cell. Antibody concentration 10 ug/ml

Applications for GTF2A1L Antibody (3E4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GTF2A1L

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. Alternative splicing for this locus has been observed and two variants, encoding distinct isoforms, have been identified. Co-transcription of this gene and the neighboring upstream gene generates a rare transcript (SALF), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

Alternate Names

1-like factor, general transcription factor IIA, 1-like, GTF2A1LF, MGC26254, TFIIA large subunit isoform ALF, TFIIA-alpha and beta-like factor, TFIIA-alpha/beta-like factor

Entrez Gene IDs

11036 (Human)

Gene Symbol

GTF2A1L

OMIM

605358 (Human)

Additional GTF2A1L Products

Product Documents for GTF2A1L Antibody (3E4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GTF2A1L Antibody (3E4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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