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GTF2H1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38556

Novus Biologicals, part of Bio-Techne
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NBP2-38556
NBP2-38556-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PEGKAKIQLQLVLHAGDTTNFHFSNESTAVKERDAVKDLLQQLLPKFKRKANKELEEKNRMLQEDPVLFQLYKDLVVSQVISAEEFWANRLNVNATDSSSTSNHKQDVGISAAFLADVRPQTDGCNGLRYN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GTF2H1 Antibody

Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556]

Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556]

Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Analysis in human testis and skeletal muscle tissues using NBP2-38556 antibody. Corresponding GTF2H1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: GTF2H1 Antibody [NBP2-38556]

Immunocytochemistry/ Immunofluorescence: GTF2H1 Antibody [NBP2-38556]

Immunocytochemistry/Immunofluorescence: GTF2H1 Antibody [NBP2-38556] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556]

Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556]

Immunohistochemistry-Paraffin: GTF2H1 Antibody [NBP2-38556] - Staining of human skeletal muscle shows no nuclear positivity in myocytes.

Applications for GTF2H1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GTF2H1

GTF2H1 is a component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNAand, when complexed to CAK, in RNA transcription by RNA polymerase II

Alternate Names

Basic transcription factor 2 62 kDa subunit, BTF2 p62, BTF2general transcription factor IIH, polypeptide 1 (62kD subunit), General transcription factor IIH polypeptide 1, general transcription factor IIH subunit 1, general transcription factor IIH, polypeptide 1, 62kDa, TFB1, TFIIH, TFIIH basal transcription factor complex p62 subunit

Gene Symbol

GTF2H1

UniProt

Additional GTF2H1 Products

Product Documents for GTF2H1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GTF2H1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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