GTF3C5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-60435

Novus Biologicals, part of Bio-Techne
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NB100-60435
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all GTF3C5 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 469 and 519 of human general transcription factor 3C polypeptide 5 (Transcription factor IIIC 63 kDa subunit) using the numbering given in entry NP_036219.1 (GeneID 9328).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GTF3C5 Antibody

Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435] - Detection of human and mouse GTF3C5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GTF3C5 antibody NB100-60435 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435]

Immunohistochemistry: GTF3C5 Antibody [NB100-60435] - Detection of human GTF3C5/TFIIIC63 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- GTF3C5/TFIIIC63 (NB100-60435). Detection: DAB
Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435]

Western Blot: GTF3C5 Antibody [NB100-60435] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NB100-60435 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.
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Applications for GTF3C5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GTF3C5

General transcription factor 3C polypeptide 5 (GTF3C5/TFIIIC63) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. GTF3C5/TFIIIC63 interacts with TFIIIB and may facilitate TFIIIB and RNA polymerase III recruitment in association with TFIIIC102, TFIIIB90, and RPC62. Alternate names for GTF3C5/TFIIIC63 include transcription factor IIIC subunit epsilon, TF3C-epsilon, TFIIIC 63 kDa subunit, general transcription factor IIIC polypeptide 5 (63 kDa), and FLJ20857.

Alternate Names

FLJ20857, general transcription factor 3C polypeptide 5, general transcription factor IIIC, polypeptide 5 (63kD), general transcription factor IIIC, polypeptide 5, 63kDa, TF3C-epsilon, TFiiiC2-63, TFIIIC63TFIIIC 63 kDa subunit, TFIIICepsilon, Transcription factor IIIC 63 kDa subunit, Transcription factor IIIC subunit epsilon, transcription factor IIIC, 63 kD

Entrez Gene IDs

9328 (Human); 70239 (Mouse)

Gene Symbol

GTF3C5

UniProt

NP_036219.1 (Human)

Additional GTF3C5 Products

Product Documents for GTF3C5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GTF3C5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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