H-2Dd Antibody (34-5-8S) [PE]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28188

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunoprecipitation

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 34-5-8S

Concentration

0.1 mg/ml

View all H-2Dd Antibodies »

Product Summary for H-2Dd Antibody (34-5-8S) [PE]

Immunogen

BDF1 mouse splenocytes

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for H-2Dd Antibody (34-5-8S) [PE]

Flow Cytometry: H-2Dd Antibody (34-5-8S) [PE] [NBP1-28188] - Analysis of DBA/2 splenocytes.

Applications for H-2Dd Antibody (34-5-8S) [PE]

Application
Recommended Usage

Flow Cytometry

0.2 ug/10^6 cells

Immunoprecipitation

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS and a stabilizing agent

Preservative

Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: H-2Dd

H-2Dd MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)

Alternate Names

H2Dd, MHC class I

Additional H-2Dd Products

Product Documents for H-2Dd Antibody (34-5-8S) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for H-2Dd Antibody (34-5-8S) [PE]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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