H4 Clustered Histone 1 [ac Lys12] Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35422
![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202414154357.jpg "Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic acetylated peptide around K12 of human H4 Clustered Histone 1 (NP_001029249.1).
Sequence:
GLGKG
Sequence:
GLGKG
Modification
ac Lys 12
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for H4 Clustered Histone 1 [ac Lys12] Antibody
![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202414154362.jpg "Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -
Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunofluorescence analysis of C6 cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202415133485.jpg "Western Blot: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
Western Blot: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -
Western Blot: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Western blot analysis of various lysates using H4 Clustered Histone 1 Rabbit pAb at 1:500 dilution. HeLa cells and C6 cells were treated by TSA (1 uM) at 37C for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202414112823.jpg "Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -
Immunocytochemistry/ Immunofluorescence: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] - Immunofluorescence analysis of NIH/3T3 cells using H4 Clustered Histone 1 Rabbit pAb at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37C for 18 hours. Blue: DAPI for nuclear staining.![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202415171180.jpg "Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202415171150.jpg "Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
![H4 Clustered Histone 1 [ac Lys12] Antibody](https://resources.bio-techne.com/images/products/nbp3-35422_rabbit-h4-clustered-histone-1-ac-lys-12-pab-912202414213745.jpg "Immunohistochemistry: H4 Clustered Histone 1 [ac Lys12] Antibody [NBP3-35422] -")
Applications for H4 Clustered Histone 1 [ac Lys12] Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: H4 Clustered Histone 1
Alternate Names
H4-16;H4C11;H4C12;H4C13;H4C14;H4C15;H4C2;H4C3;H4C4;H4C5;H4C6;H4C8;H4C9;H4FA, H4F4, HIST1H4A
Gene Symbol
H4C1
Additional H4 Clustered Histone 1 Products
Product Documents for H4 Clustered Histone 1 [ac Lys12] Antibody
Product Specific Notices for H4 Clustered Histone 1 [ac Lys12] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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