HA Tag Antibody (RMH02) - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61476

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Sandwich ELISA Capture, Sandwich ELISA Detection, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # RMH02

Concentration

1 mg/ml

View all HA Tag Antibodies »

Product Specifications

Immunogen

This HA Tag Antibody (RMH02) was developed against a peptide corresponding to HA Tag (Human influenza hemagglutinin amino acids 98-106). This antibody is a Rabbit-Human Chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of NBP2-61477 with the constant domain of Human IgG1. This antibody is recognized only by an anti-Human IgG secondary antibody.

Specificity

This antibody reacts to recombinant proteins containing a HA Tag fused to either the amino or carboxy terminus. No cross reactivity with any endogenous protein.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for HA Tag Antibody (RMH02) - Chimeric

Western Blot: HA Tag Antibody (RMH02)Chimeric [NBP2-61476]

Western Blot: HA Tag Antibody (RMH02)Chimeric [NBP2-61476]

Western Blot: HA Tag Antibody (RMH02) [NBP2-61476] - Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using NBP2-61476, followed by a HRP conjugated anti-Human IgG secondary antibody.
ELISA: HA Tag Antibody (RMH02) - Chimeric [NBP2-61476]

ELISA: HA Tag Antibody (RMH02) - Chimeric [NBP2-61476]

ELISA: HA Tag Antibody (RMH02) [NBP2-61476] - Sandwich ELISA performed on whole lysate of LNCaP cells overexpressing HA-tag Bag1 protein. ELISA plate was coated with chimeric human anti-HA-Tag antibody Clone NBP2-61476 as the capture antibody. Anti-Bag1 rabbit serum was used as the detection antibody, followed by an alkaline phosphatase conjugated anti-Rabbit IgG as the Secondary antibody.

Applications for HA Tag Antibody (RMH02) - Chimeric

Application
Recommended Usage

Sandwich ELISA Capture

0.5 - 2 ug/ml

Sandwich ELISA Detection

0.01 - 0.5 ug/ml

Western Blot

0.1 ug/ml - 1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HA Tag

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Alternate Names

HA Epitope Tag

Additional HA Tag Products

Product Documents for HA Tag Antibody (RMH02) - Chimeric

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HA Tag Antibody (RMH02) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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