HA Tag Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16773
Key Product Details
Species Reactivity
Epitope Tag
Applications
ELISA, Western Blot
Label
HRP
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to HA Tag
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HA Tag Antibody [HRP]
Western Blot: HA Tag Antibody [HRP] [NBP2-16773] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HA Tag antibody (HRP) diluted at 1:3000.
ELISA: HA Tag Antibody [HRP] [NBP2-16773] - An ELISA plate is coated with 50 uL of a DDDDK Tag conjugated BSA at concentration of 5 ug/mL. The peptide conjugated carrier protein is coated and detected with HRP-conjugated anti-DDDDK Tag antibody at concentration ranged from 3.1 to 200 ng/mL.
Applications for HA Tag Antibody [HRP]
Application
Recommended Usage
ELISA
1:1000 - 1:10000
Western Blot
1:500 - 1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: HA Tag
References
1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076
2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778
3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093
4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73
5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107
Alternate Names
HA Epitope Tag
Additional HA Tag Products
Product Specific Notices for HA Tag Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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