HA95/AKAP8L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47440

Novus Biologicals, part of Bio-Techne
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NBP2-47440
NBP2-47440-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (92%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HA95/AKAP8L Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DEEGKEDGREEGKEDPEKGALTTQDENGQTKRKLQAGKKSQDKQKKRQRDRMVERIQFVCSLCKYRTFYEDEMASHLDSKFHKEHFKYVGTKLPKQTAD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HA95/AKAP8L Antibody

Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440]

Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440]

Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining in human testis and liver tissues using anti-AKAP8L antibody. Corresponding AKAP8L RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440]

Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440]

Immunohistochemistry-Paraffin: HA95/AKAP8L Antibody [NBP2-47440] - Staining of human cerebral cortex, colon, kidney and testis using Anti-AKAP8L antibody NBP2-47440 (A) shows similar protein distribution across tissues to independent antibody NBP2-47441 (B).
Western Blot: HA95/AKAP8L Antibody [NBP2-47440]

Western Blot: HA95/AKAP8L Antibody [NBP2-47440]

Western Blot: HA95/AKAP8L Antibody [NBP2-47440] - Analysis in human cell line RT-4 and human cell line U-251 MG.
View 6 more images

Applications for HA95/AKAP8L Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICCIF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HA95/AKAP8L

AKAP8L/HA95 is a PKA anchoring protein that is localized to the nuclear envelope and associates with chromatin upon nuclear envelope breakdown during mitosis. AKAP8L/HA95 has been found to play an important role in the initiation of DNA replication and may function as a scaffold for proteins such as MCM2 and LAP2beta. Additionally, AKAP8L/HA95 has been shown to co-locate with the PKA C subunit in splicing factor compartments and is involved in the regulation of pre-mRNA splicing. Further support for a scaffolding and splicing role in the nucleus comes from the observation that AKAP8L/HA95 colocalizes with HypA, a splicing-like factor, and through this association may function as a docking site for the nuclear accumulation of Huntington protein as seen in Huntington's disease.

Alternate Names

A kinase (PRKA) anchor protein 8-like, AKAP8-like protein, HA95, HAP95 DKFZp434L0650, helicase A-binding protein 95 kDa, Homologous to AKAP95 protein, HRIHFB2018, NAKAP, NAKAP95 A-kinase anchor protein 8-like, neighbor of A kinase anchoring protein 95, Neighbor of AKAP95, Neighbor of A-kinase-anchoring protein 95

Gene Symbol

AKAP8L

Additional HA95/AKAP8L Products

Product Documents for HA95/AKAP8L Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HA95/AKAP8L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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