Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HADH Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 57-314 of human HADH (NP_005318) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HADH Antibody (OTI3D12) - Azide and BSA Free

Western Blot: HADH Antibody (OTI3D12)Azide and BSA Free [NBP2-70875]

Western Blot: HADH Antibody (OTI3D12)Azide and BSA Free [NBP2-70875]

Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HT29 cell lysate (35ug) by using HADH monoclonal antibody.
Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875]

Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875]

Immunohistochemistry: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: HADH Antibody (OTI3D12)Azide and BSA Free [NBP2-70875]

Western Blot: HADH Antibody (OTI3D12)Azide and BSA Free [NBP2-70875]

Western Blot: HADH Antibody (OTI3D12) - Azide and BSA Free [NBP2-70875] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HADH.
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Applications for HADH Antibody (OTI3D12) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HADH

HADH is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene.

Alternate Names

EC 1.1.1, EC 1.1.1.35, HADH1, HADHSChydroxyacyl-Coenzyme A dehydrogenase, HCDH, hydroxyacyl-CoA dehydrogenase, L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain, Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase, MGC8392, mitochondrial, MSCHAD, SCHADHHF4

Gene Symbol

HADH

Additional HADH Products

Product Documents for HADH Antibody (OTI3D12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HADH Antibody (OTI3D12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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