HAP1 Antibody (1B6)
Novus Biologicals, part of Bio-Techne | Catalog # NB110-74569
![Western Blot: HAP1 Antibody (1B6) [NB110-74569]](https://resources.bio-techne.com/images/products/HAP1-Antibody-1B6-Western-Blot-NB110-74569-img0005.jpg "Western Blot: HAP1 Antibody (1B6) [NB110-74569]")
![Western Blot: HAP1 Antibody (1B6) [NB110-74569]](https://resources.bio-techne.com/images/products/HAP1-Antibody-1B6-Western-Blot-NB110-74569-img0006.jpg "Western Blot: HAP1 Antibody (1B6) [NB110-74569]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1B6
Concentration
1 mg/ml
Product Specifications
Immunogen
Fusion protein from rat HAP1. [UniProt# P54256]
Specificity
This is specific for isoforms A and B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for HAP1 Antibody (1B6)
Western Blot: HAP1 Antibody (1B6) [NB110-74569]
Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.![Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]](https://resources.bio-techne.com/images/products/HAP1-Antibody-1B6-Immunohistochemistry-NB110-74569-img0004.jpg "Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]")
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.![Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]](https://resources.bio-techne.com/images/products/HAP1-Antibody-1B6-Flow-Cytometry-NB110-74569-img0007.jpg "Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]")
Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]
Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.Applications for HAP1 Antibody (1B6)
Application
Recommended Usage
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
reported in scientific literature
Immunohistochemistry
0.5-1.0 ug/ml
Immunohistochemistry-Frozen
0.5-1.0 ug/ml
Immunohistochemistry-Paraffin
0.5-1.0 ug/ml
Immunoprecipitation
1:10-1:500
Western Blot
0.5-1.0 ug/ml
Application Notes
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NB110-74569 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS and 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HAP1
Alternate Names
HAP2huntingtin-associated protein 2, hHLP1, HIP5, HLP1, HLPHAP-1, huntingtin-associated protein 1, Neuroan 1
Gene Symbol
HAP1
Additional HAP1 Products
Product Documents for HAP1 Antibody (1B6)
Product Specific Notices for HAP1 Antibody (1B6)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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