HAP1 Antibody (1B6)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-74569

Novus Biologicals, part of Bio-Techne
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NB110-74569
NB110-74569SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1B6

Concentration

1 mg/ml

View all HAP1 Antibodies »

Product Specifications

Immunogen

Fusion protein from rat HAP1. [UniProt# P54256]

Specificity

This is specific for isoforms A and B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HAP1 Antibody (1B6)

Western Blot: HAP1 Antibody (1B6) [NB110-74569]

Western Blot: HAP1 Antibody (1B6) [NB110-74569]

Western Blot: HAP1 Antibody (1B6) [NB110-74569] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate.
Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]

Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569]

Immunohistochemistry: HAP1 Antibody (1B6) [NB110-74569] - Staining of rat hypothalamus.
Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]

Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569]

Flow Cytometry: HAP1 Antibody (1B6) [NB110-74569] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Applications for HAP1 Antibody (1B6)

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunohistochemistry

0.5-1.0 ug/ml

Immunohistochemistry-Frozen

0.5-1.0 ug/ml

Immunohistochemistry-Paraffin

0.5-1.0 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

0.5-1.0 ug/ml
Application Notes
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.

Reviewed Applications

Read 1 review rated 5 using NB110-74569 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HAP1

HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.

Alternate Names

HAP2huntingtin-associated protein 2, hHLP1, HIP5, HLP1, HLPHAP-1, huntingtin-associated protein 1, Neuroan 1

Gene Symbol

HAP1

Additional HAP1 Products

Product Documents for HAP1 Antibody (1B6)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HAP1 Antibody (1B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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