HARS Antibody (1C8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003035-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1C8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HARS Antibodies »

Product Specifications

Immunogen

HARS (NP_002100, 1 a.a. ~ 96 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPV

Specificity

HARS - histidyl-tRNA synthetase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HARS Antibody (1C8)

Western Blot: HARS Antibody (1C8) [H00003035-M01]

Western Blot: HARS Antibody (1C8) [H00003035-M01]

Western Blot: HARS Antibody (1C8) [H00003035-M01] - HARS monoclonal antibody (M01), clone 1C8 Analysis of HARS expression in HeLa.
Immunocytochemistry/ Immunofluorescence: HARS Antibody (1C8) [H00003035-M01]

Immunocytochemistry/ Immunofluorescence: HARS Antibody (1C8) [H00003035-M01]

Immunocytochemistry/Immunofluorescence: HARS Antibody (1C8) [H00003035-M01] - Analysis of monoclonal antibody to HARS on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: HARS Antibody (1C8) [H00003035-M01] - Detection limit for recombinant GST tagged HARS is approximately 0.03ng/ml as a capture antibody.

Applications for HARS Antibody (1C8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HARS

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

Alternate Names

cytoplasmic, EC 6.1.1.21, FLJ20491, histidine-tRNA ligase, histidyl-tRNA synthetase, histidyl-tRNA synthetase, cytoplasmic

Entrez Gene IDs

3035 (Human)

Gene Symbol

HARS1

OMIM

142810 (Human)

UniProt

NP_002100 (Human)

Additional HARS Products

Product Documents for HARS Antibody (1C8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HARS Antibody (1C8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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