HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12352

Novus Biologicals, part of Bio-Techne
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NBP2-12352-0.025mg
NBP2-12352
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Block/Neutralize, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2 Clone # MM0324-3R44

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all HB-EGF Antibodies »

Product Summary for HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free

Immunogen

Human recombinant HB-EGF

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2

Scientific Data Images for HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free

Immunohistochemistry-Paraffin: HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free [NBP2-12352]

Immunohistochemistry-Paraffin: HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free [NBP2-12352]

Immunohistochemistry-Paraffin: HB-EGF Antibody (MM0324-3R44) [NBP2-12352] - 10% Buffer formalin fixed and parffine embedded human placetal tissue section (4um) is subjected IHC staining using NBP2-12352. Tissue section was pretreated in citric buffer (pH 6.0) with microwave for antige retrieval before IHC is applied.

Applications for HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:1000
Application Notes
This antibody has been validated to neutralize HB-EGF bioactivity.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.5 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HB-EGF

HB-EGF is an EGF related growth factor that signals through the EGF receptor, and stimulates the proliferation of smooth muscle cells (SMC), fibroblasts, epithelial cells, and keratinocytes. HB-EGF is expressed in numerous cell types and tissues, including vascular endothelial cells and SMC, macrophages, skeletal muscle, keratinocytes, and certain tumor cells. The ability of HB-EGF to specifically bind heparin and heparin sulfate proteoglycans is distinct from other EGF-like molecules, and may be related to the enhanced mitogenic activity, relative to EGF, that HB-EGF exerts on smooth muscle cells. The human HB-EGF gene encodes a 208 amino acid transmembrane protein, which can be proteolytically cleaved to produce soluble HB-EGF. Recombinant human HB-EGF is a 9.7 kDa protein containing 86 amino acid residues, corresponding to the extra-cellular EGF-like and heparin binding domains of the full length HB-EGF protein.

Long Name

Heparin Binding EGF-like Growth Factor

Alternate Names

Dtr, Dts, HBEGF, Hegfl

Gene Symbol

HBEGF

UniProt

Q53H93

Additional HB-EGF Products

Product Documents for HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HB-EGF Antibody (MM0324-3R44) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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