Key Product Details

Species Reactivity

Virus

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # HB5

Format

BSA Free

Concentration

1 mg/ml

View all HBsAg Antibodies »

Product Specifications

Immunogen

purified HbsAg from human plasma

Specificity

This antibody (clone HB5) recognizes following Hepatitis B virus (HBV) subtypes: ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Hepatitis B surface antigen (HBsAg) is a marker of infectivity. Specificity of the antibody HB5 was verified by ELISA on panel of virus subtypes identified on International Workshop on HBsAg Subtypes (Paris, April 1975). The antibody HB5 does not cross-block with This antibody HB3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for HBsAg Antibody (HB5) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000
Application Notes
ELISA: Use at an assay dependant concentration. ICC: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HBsAg

Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

Alternate Names

HBsAg, HBV major surface antigen, HBV surface antigen, Hepatitis B Virus major surface antigen, Hepatitis B Virus Surface Antigen, Major surface antigen, protein S

Gene Symbol

S

Additional HBsAg Products

Product Documents for HBsAg Antibody (HB5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HBsAg Antibody (HB5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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