HCA59 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83168

Novus Biologicals, part of Bio-Techne
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NBP1-83168
NBP1-83168-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HCA59 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MPVVRKIFRRRRGDSESEEDEQDSEEVRLKLEETREVQNLRKRPNGVSAVALLVGEKVQEETTLVDDPFQMKTGGMVDMKKLKE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23276153)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HCA59 Antibody

Western Blot: HCA59 Antibody [NBP1-83168]

Western Blot: HCA59 Antibody [NBP1-83168]

Western Blot: HCA59 Antibody [NBP1-83168] - Analysis using Anti-C9orf78 antibody NBP1-83168 (A) shows similar pattern to independent antibody NBP1-83170 (B).
Immunocytochemistry/ Immunofluorescence: HCA59 Antibody [NBP1-83168]

Immunocytochemistry/ Immunofluorescence: HCA59 Antibody [NBP1-83168]

Immunocytochemistry/Immunofluorescence: HCA59 Antibody [NBP1-83168] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]

Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168]

Immunohistochemistry-Paraffin: HCA59 Antibody [NBP1-83168] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
View 15 more images

Applications for HCA59 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HCA59

Alternate Names

bA409K20.3, C9orf78, chromosome 9 open reading frame 78, HCA59, Hepatocellular carcinoma-associated antigen 59, HSPC220

Gene Symbol

C9ORF78

Additional HCA59 Products

Product Documents for HCA59 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HCA59 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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