Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all HCC1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 325-375 of human Caper, Coactivator of activating protein-1 and estrogen receptors using the SwissProt entry Q14498 (GeneID 9584).

Reactivity Notes

Orangutan (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HCC1 Antibody - BSA Free

Western Blot: HCC1 AntibodyBSA Free [NB200-333]

Western Blot: HCC1 AntibodyBSA Free [NB200-333]

Western Blot: HCC1 Antibody [NB200-333] - Detection of human Caper by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Caper antibody NB200-333 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: HCC1 Antibody - BSA Free [NB200-333]

Immunohistochemistry-Paraffin: HCC1 Antibody - BSA Free [NB200-333]

Immunohistochemistry-Paraffin: HCC1 Antibody [NB200-333] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
Immunoprecipitation: HCC1 Antibody - BSA Free [NB200-333]

Immunoprecipitation: HCC1 Antibody - BSA Free [NB200-333]

Immunoprecipitation: HCC1 Antibody [NB200-333] - Detection of human HCC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HCC1 antibody NB200-333 (lot 2) used for IP at 6 ug per reaction. HCC1 was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated HCC1, NB200-333 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for HCC1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Phosphate Buffered Saline (PBS)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HCC1

Transcriptional coactivators either bridge transcription factors and the components of the basal transcription apparatus and/or remodel the chromatin structures. CAPER (coactivator of activating protein-1 (AP-1) and estrogen receptors (ERs)) may represent a selective transcriptional coactivator molecule that plays a pivotal role for the function of AP-1 and ERs in vivo in conjunction with the general coactivator ASC-2.

Long Name

RNA-binding protein 39

Alternate Names

CAPER alpha, CAPERalpha, RBM39, RNPC2

Entrez Gene IDs

9584 (Human)

Gene Symbol

RBM39

UniProt

Q14498 (Human)

Additional HCC1 Products

Product Documents for HCC1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HCC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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