HCCS Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86577
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYEL
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25255805).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HCCS Antibody
Western Blot: HCCS Antibody [NBP1-86577]
Western Blot: HCCS Antibody [NBP1-86577] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).Immunocytochemistry/ Immunofluorescence: HCCS Antibody [NBP1-86577]
Immunocytochemistry/Immunofluorescence: HCCS Antibody [NBP1-86577] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.Immunohistochemistry-Paraffin: HCCS Antibody [NBP1-86577]
Immunohistochemistry-Paraffin: HCCS Antibody [NBP1-86577] - Staining of human parathyroid gland shows strong cytoplasmic positivity in glandular cells.Applications for HCCS Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25255805). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HCCS
Alternate Names
CCHLDKFZp779I1858, cytochrome c heme-lyase, cytochrome c-type heme lyase, EC 4.4.1.17, holocytochrome c synthase, holocytochrome c synthase (cytochrome c heme-lyase), Holocytochrome c-type synthase, MCOPS7
Gene Symbol
HCCS
Additional HCCS Products
Product Documents for HCCS Antibody
Product Specific Notices for HCCS Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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