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HCCS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86577

Novus Biologicals, part of Bio-Techne
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NBP1-86577
NBP1-86577-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YVECPIRGTAAENKENLDPSNLMPPPNQTPAPDQPFALSTVREESSIPRADSEKKWVYPSEQMFWNAMLKKGWKWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSLIRFGGKAKEYSPRARIRSWMGYEL

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25255805).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HCCS Antibody

Western Blot: HCCS Antibody [NBP1-86577]

Western Blot: HCCS Antibody [NBP1-86577]

Western Blot: HCCS Antibody [NBP1-86577] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: HCCS Antibody [NBP1-86577]

Immunocytochemistry/ Immunofluorescence: HCCS Antibody [NBP1-86577]

Immunocytochemistry/Immunofluorescence: HCCS Antibody [NBP1-86577] - Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: HCCS Antibody [NBP1-86577]

Immunohistochemistry-Paraffin: HCCS Antibody [NBP1-86577]

Immunohistochemistry-Paraffin: HCCS Antibody [NBP1-86577] - Staining of human parathyroid gland shows strong cytoplasmic positivity in glandular cells.

Applications for HCCS Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 25255805). For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HCCS

Links covalently the heme group to the apoprotein of cytochrome c

Alternate Names

CCHLDKFZp779I1858, cytochrome c heme-lyase, cytochrome c-type heme lyase, EC 4.4.1.17, holocytochrome c synthase, holocytochrome c synthase (cytochrome c heme-lyase), Holocytochrome c-type synthase, MCOPS7

Gene Symbol

HCCS

Additional HCCS Products

Product Documents for HCCS Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HCCS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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