HCN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12563

Novus Biologicals, part of Bio-Techne
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NBP3-12563-100ul
NBP3-12563-50ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HCN1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human HCN1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for HCN1 Antibody

Western Blot: HCN1 Antibody [NBP3-12563]

Western Blot: HCN1 Antibody [NBP3-12563]

Western Blot: HCN1 Antibody [NBP3-12563] - Lane A: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:99 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563]

Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563]

Immunohistochemistry-Paraffin: HCN1 Antibody [NBP3-12563] - Staining of human HCN1 in human brain with rabbit polyclonal antibody at 1:500 dilution.
Immunoprecipitation: HCN1 Antibody [NBP3-12563]

Immunoprecipitation: HCN1 Antibody [NBP3-12563]

Immunoprecipitation: HCN1 Antibody [NBP3-12563] - Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-HCN1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-HCN1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 99 kDaObserved band size :100 kDa

Applications for HCN1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HCN1

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulations of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (3).

Long Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Alternate Names

BCNG-1, BCNG1, EIEE24, GEFSP10, HAC-2

Gene Symbol

HCN1

Additional HCN1 Products

Product Documents for HCN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HCN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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