HCN1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13014

Novus Biologicals, part of Bio-Techne
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NBP3-13014
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Rabbit (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HCN1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human HCN1. The exact sequence is proprietary.

Reactivity Notes

Mouse (88%), Rat (84%), Bovine (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for HCN1 Antibody

Western Blot: HCN1 Antibody [NBP3-13014]

Western Blot: HCN1 Antibody [NBP3-13014]

Western Blot: HCN1 Antibody [NBP3-13014] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HCN1 antibody (NBP3-13014) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: HCN1 Antibody [NBP3-13014]

Immunocytochemistry/ Immunofluorescence: HCN1 Antibody [NBP3-13014]

Immunocytochemistry/Immunofluorescence: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HCN1 stained by HCN1 antibody (NBP3-13014) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014]

Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014]

Immunohistochemistry-Frozen: HCN1 Antibody [NBP3-13014] - HCN1 antibody detects HCN1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: HCN1 protein stained by HCN1 antibody (NBP3-13014) diluted at 1:250. Blue: Fluoroshield with DAPI.
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Applications for HCN1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HCN1

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, play a crucial role in the regulations of cell excitability. Importantly, they contribute to spontaneous rhythmic activity in both the heart and brain (3).

Long Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Alternate Names

BCNG-1, BCNG1, EIEE24, GEFSP10, HAC-2

Gene Symbol

HCN1

Additional HCN1 Products

Product Documents for HCN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HCN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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