Key Product Details

Species Reactivity

Human, Mouse, Rat, Rabbit

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S114/10

Concentration

1 mg/ml

View all HCN4 Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1019-1198 (c-terminus) of rat HCN4

Localization

Membrane

Specificity

Detects approx 130 kDa. No cross-reactivity against other HCNs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HCN4 Antibody (S114/10)

Western Blot: HCN4 Antibody (S114/10) [NBP2-12910]

Western Blot: HCN4 Antibody (S114/10) [NBP2-12910]

Western Blot: HCN4 Antibody (S114/10) [NBP2-12910] - Western Blot analysis of Human T-HEK cell lysate showing detection of HCN4 protein using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Immunocytochemistry/ Immunofluorescence: HCN4 Antibody (S114/10) [NBP2-12910]

Immunocytochemistry/ Immunofluorescence: HCN4 Antibody (S114/10) [NBP2-12910]

Immunocytochemistry/Immunofluorescence: HCN4 Antibody (S114/10) [NBP2-12910] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN4 Antibody (D) Composite.
Immunohistochemistry: HCN4 Antibody (S114/10) [NBP2-12910]

Immunohistochemistry: HCN4 Antibody (S114/10) [NBP2-12910]

Immunohistochemistry: HCN4 Antibody (S114/10) [NBP2-12910] - Immunohistochemistry analysis using Mouse Anti-HCN4 Monoclonal Antibody, Clone N114/10 (NBP2-12910). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN4 Monoclonal Antibody (NBP2-12910) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
View 1 more images

Applications for HCN4 Antibody (S114/10)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:1000

Western Blot

1:1000
Application Notes
1 ug/mL of HCN4 Antibody was sufficient for detection of HCN4 in 10 ug of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HCN4

1 ug/mL was sufficient for detection of HCN4 in 10ug of COS cell lysate transiently transfected with HCN4 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Long Name

Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 4

Alternate Names

SSS2

Gene Symbol

HCN4

Additional HCN4 Products

Product Documents for HCN4 Antibody (S114/10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HCN4 Antibody (S114/10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.