HDAC3 Antibody (PCRP-HDAC3-2D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13809

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-HDAC3-2D4

Concentration

0.2 mg/ml

View all Histone Deacetylase 3/HDAC3 Antibodies »

Product Summary for HDAC3 Antibody (PCRP-HDAC3-2D4)

Immunogen

Recombinant full-length human HDAC3 protein (Uniprot: O15379)

Localization

Nucleus, Nucleoplasm, Golgi apparatus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14060)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for HDAC3 Antibody (PCRP-HDAC3-2D4)

Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809]

Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809]

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC3 Antibody (PCRP-HDAC3-2D4) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).
Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809]

Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809]

Flow Cytometry: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC3 antibody (PCRP-HDAC3-2D4) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809]

Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809]

Protein Array: HDAC3 Antibody (PCRP-HDAC3-2D4) [NBP3-13809] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC3 Antibody (PCRP-HDAC3-2D4).

Applications for HDAC3 Antibody (PCRP-HDAC3-2D4)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein
Application Notes
ELISA: For coating, order antibody without BSA

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Gene Symbol

HDAC3

UniProt

O15379 (Human)

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody (PCRP-HDAC3-2D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC3 Antibody (PCRP-HDAC3-2D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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