HDAC3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-126

Novus Biologicals, part of Bio-Techne
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NB500-126
NB500-126-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

12.5 mg/ml

View all Histone Deacetylase 3/HDAC3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding to amino acids 411-428 of human and chicken HDAC3.

Epitope

The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.

Localization

Nuclear

Specificity

Anti-Histone Deacetylase 3 specifically recognizes histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kD).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HDAC3 Antibody - BSA Free

Western Blot: HDAC3 AntibodyBSA Free [NB500-126]

Western Blot: HDAC3 AntibodyBSA Free [NB500-126]

Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK
Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126]

Immunocytochemistry/ Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126]

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126]

Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126]

Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126] - Formalin-fixed, paraffin-embedded section of lymph node stained with 1:500 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in Rabbit followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by ExtrAvidin(R)-Peroxidase

Applications for HDAC3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500

Immunoprecipitation

1:2500

Western Blot

1:2000-1:10000
Application Notes
An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

12.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 3/HDAC3

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone cetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.

Long Name

Histone Deacetylase 3

Alternate Names

HDAC3, KDAC3, RPD3-2, SMAP45

Entrez Gene IDs

8841 (Human); 15183 (Mouse); 84578 (Rat)

Gene Symbol

HDAC3

UniProt

O15379 (Human)

Additional Histone Deacetylase 3/HDAC3 Products

Product Documents for HDAC3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HDAC3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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