HDAC7 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44092
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HDAC7 Antibody
Immunogen
HDAC7 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N terminal region of human HDAC7. (Uniprot: Q8WUI4)
Reactivity Notes
Cross-reactivity with HDAC7 from other sources has not been determined
Localization
Cell nucleus, Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for HDAC7 Antibody
![Western Blot: HDAC7 Antibody [NBP1-44092]](https://resources.bio-techne.com/images/products/HDAC7-Antibody-Western-Blot-NBP1-44092-img0003.jpg "Western Blot: HDAC7 Antibody [NBP1-44092]")
Western Blot: HDAC7 Antibody [NBP1-44092]
Western Blot: HDAC7 Antibody [NBP1-44092] - Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 ug per lane. Primary antibody: HDAC7 antibody at 1ug/mL for overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 103kDa/ 103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms 70 and 40kDa.Applications for HDAC7 Antibody
Application
Recommended Usage
ELISA
1:20000-1:60000
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1 ug/ml
Application Notes
This product has been tested by ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~103kDa and isoforms at ~70, and 40 kDa corresponding to the appropriate cell lysate or extract.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using NBP1-44092 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: HDAC7
Long Name
Histone deacetylase 7
Alternate Names
HD7, HD7a, HDAC7A
Entrez Gene IDs
51564 (Human)
Gene Symbol
HDAC7
UniProt
Additional HDAC7 Products
Product Documents for HDAC7 Antibody
Product Specific Notices for HDAC7 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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