Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot (Negative)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HECTD1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 2100 and 2150 of human HECT Domain Containing 1 using the numbering given in entry AAP13073.1 (GeneID 25831).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HECTD1 Antibody

Western Blot: HECTD1 Antibody [NBP1-49926]

Western Blot: HECTD1 Antibody [NBP1-49926]

Western Blot: HECTD1 Antibody [NBP1-49926] - USP15 knockdown with three siRNAs in LN-229 and (b) two siRNAs in LN-428. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798) licensed under a CC-BY license. USP15 antibody (catalog # NB110-40690)
Immunoprecipitation: HECTD1 Antibody [NBP1-49926]

Immunoprecipitation: HECTD1 Antibody [NBP1-49926]

Immunoprecipitation: HECTD1 Antibody [NBP1-49926] - Detection of Human HECTD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HECTD1 antibody used for IP at 3 mcg/mg lysate. HECTD1 was also immunoprecipitated by rabbit anti-HECTD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated HECTD1 was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
HECTD1 Antibody

Western Blot: HECTD1 Antibody [NBP1-49926] -

USP15 knockdown leads to decreased HECTD1 protein levels(a) USP15 knockdown with three siRNAs in LN-229 & (b) two siRNAs in LN-428. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.22798), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
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Applications for HECTD1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HECTD1

The HECTD1 gene encodes a2, 610 amino acid long, 298 kDA E3 ubiquitin-protein ligase HECTD1 protein that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester. It then directly transmits the ubiquitin to targeted substrates and may be essential for development of the head mesenchyme and neural tube closure. HECTD1 interacts in interferon signaling and cytokine signaling in the immune system and is known to interact with various genes such as NME2, ERCC2, PITX2, CCNA2, and NME1-NME2. HECTD1 has been investigated in many diseases such as angelman syndrome, teratoma, carcinoma, malaria, and influenza.

Alternate Names

E3 ligase for inhibin receptor, E3 ubiquitin-protein ligase HECTD1, EC 6.3.2.-, EULIR, HECT domain containing 1, HECT domain-containing protein 1, KIAA1131FLJ38315

Entrez Gene IDs

25831 (Human)

Gene Symbol

HECTD1

UniProt

Q9ULT8 (Human)

Additional HECTD1 Products

Product Documents for HECTD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HECTD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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