Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG Clone # 22F6

Format

BSA Free

Concentration

1 mg/ml

View all Helios Antibodies »

Product Summary for Helios Antibody (22F6) - BSA Free

Immunogen

Peptide coresponding to the amino acids 51-107 of Helios.

Specificity

The armenian hamster monoclonal antibody 22F6 recognizes Helios, a transcription factor expressed in some hematopoietic stem cells, and at high levels in thymic-derived regulatory T cells.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG

Scientific Data Images for Helios Antibody (22F6) - BSA Free

Flow Cytometry: Helios Antibody (22F6) - BSA Free [NBP2-37723]

Flow Cytometry: Helios Antibody (22F6) - BSA Free [NBP2-37723]

Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Staining of CD4+ human peripheral blood lymphocytes using anti-Helios (clone 22F6) PE-CyTM7.
Flow Cytometry: Helios Antibody (22F6) - BSA Free [NBP2-37723]

Flow Cytometry: Helios Antibody (22F6) - BSA Free [NBP2-37723]

Flow Cytometry: Helios Antibody (22F6) [NBP2-37723] - Human peripheral blood lymphocytes were stained with anti-CD4 Pacific BlueTM, fixed and permeabilized with Treg Detection Kit and then intracellulary stained with anti-Helios PE (clone 22F6).

Applications for Helios Antibody (22F6) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Helios

HELIOS encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

Long Name

DNA-binding Protein Helios

Alternate Names

IKZF2, ZNF1A2, ZNFN1A2

Gene Symbol

IKZF2

Additional Helios Products

Product Documents for Helios Antibody (22F6) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Helios Antibody (22F6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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