Hemoglobin beta Antibody (7B12)

Novus Biologicals, part of Bio-Techne | Catalog # H00003043-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7B12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Hemoglobin beta Antibodies »

Product Specifications

Immunogen

HBB (AAH07075, 38 a.a. ~ 147 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. WTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALPHKYH

Specificity

HBB - hemoglobin, beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hemoglobin beta Antibody (7B12)

Immunohistochemistry-Paraffin: Hemoglobin beta Antibody (7B12) [H00003043-M02]

Immunohistochemistry-Paraffin: Hemoglobin beta Antibody (7B12) [H00003043-M02]

Immunohistochemistry-Paraffin: Hemoglobin beta Antibody (7B12) [H00003043-M02] - Analysis of monoclonal antibody to HBB on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
ELISA: Hemoglobin beta Antibody (7B12) [H00003043-M02]

ELISA: Hemoglobin beta Antibody (7B12) [H00003043-M02]

ELISA: Hemoglobin beta Antibody (7B12) [H00003043-M02] - Detection limit for recombinant GST tagged HBB is approximately 0.1ng/ml as a capture antibody.

Applications for Hemoglobin beta Antibody (7B12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hemoglobin beta

The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'.

Alternate Names

beta globin chain, beta-globin, CD113t-C, HBBB, HBD, Hemoglobin beta chain, hemoglobin subunit beta, hemoglobin, beta

Entrez Gene IDs

3043 (Human)

Gene Symbol

HBB

OMIM

141900 (Human)

UniProt

AAH07075 (Human)

Additional Hemoglobin beta Products

Product Documents for Hemoglobin beta Antibody (7B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Hemoglobin beta Antibody (7B12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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