Hemoglobin zeta Antibody (1G10)

Novus Biologicals, part of Bio-Techne | Catalog # H00003050-M03

Novus Biologicals, part of Bio-Techne
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H00003050-M03
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1G10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Hemoglobin zeta Antibodies »

Product Specifications

Immunogen

HBZ (NP_005323, 1 a.a. ~ 81 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSLTKTERTIIVSMWAKISTQADTIGTETLERLFLSHPQTKTYFPHFDLHPGSAQLRAHGSKVVAAVGDAVKSIDDIGGAL

Specificity

HBZ (1G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hemoglobin zeta Antibody (1G10)

Western Blot: Hemoglobin zeta Antibody (1G10) [H00003050-M03]

Western Blot: Hemoglobin zeta Antibody (1G10) [H00003050-M03]

Western Blot: Hemoglobin zeta Antibody (1G10) [H00003050-M03] - Analysis of HBZ expression in transfected 293T cell line by HBZ monoclonal antibody (M03), clone 1G10. Lane 1: HBZ transfected lysatE (15.6 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Hemoglobin zeta Antibody (1G10) [H00003050-M03]

Immunohistochemistry-Paraffin: Hemoglobin zeta Antibody (1G10) [H00003050-M03]

Immunohistochemistry-Paraffin: Hemoglobin zeta Antibody (1G10) [H00003050-M03] - Analysis of monoclonal antibody to HBZ on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml
Sandwich ELISA: Hemoglobin zeta Antibody (1G10) [H00003050-M03] - Detection limit for recombinant GST tagged HBZ is approximately 10ng/ml as a capture antibody.

Applications for Hemoglobin zeta Antibody (1G10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA and IP.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Hemoglobin zeta

Zeta-globin is an alpha-like hemoglobin. The zeta-globin polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult like. The zeta-globin gene is a member of the human alpha-globin gene cluster that includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'.

Alternate Names

HBAZ, HBZ, HBZ2, zeta-globin

Entrez Gene IDs

3050 (Human)

Gene Symbol

HBZ

OMIM

142310 (Human)

UniProt

NP_005323 (Human)

Additional Hemoglobin zeta Products

Product Documents for Hemoglobin zeta Antibody (1G10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hemoglobin zeta Antibody (1G10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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