Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23523
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Bovine
Cited:
Human
Applications
Validated:
ELISA, Immunoaffinity Purification, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Cytometric Bead Assay Standard
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # A74
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Bovine corneal endothelial cells
Epitope
Epitope is located in domain V
Specificity
Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
ELISA: NBP2-23523 can be used in ELISA. WB: In Western blotting a dilution guideline of 1/100 has proved successful. (1) IHC: NBP2-23523 can be used in immunostaining of frozen PLP-fixed sections of bovine and human tissues. AP: NBP2-23523 can be used in affinity chromatography, in conjunction with NBP2-23522 to separate recombinant domain I from full length perlecan. (1) IP: NBP2-23523 can be used in immunoprecipitation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM Phosphate (pH 7.4) and 0.5M NaCl
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Heparan Sulfate Proteoglycan 2
Alternate Names
basement membrane-specific heparan sulfate proteoglycan core protein, endorepellin (domain V region), heparan sulfate proteoglycan 2, HSPG, perlecan, perlecan proteoglycan, PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia), PRCAN, SJA, SJS, SJS1
Gene Symbol
HSPG2
Additional Heparan Sulfate Proteoglycan 2 Products
Product Documents for Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free
Product Specific Notices for Heparan Sulfate Proteoglycan 2 Antibody (A74) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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