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Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25466

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25466

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2317

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Immunogen

Recombinant fragment of human Hepassocin/FGL1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Western Blot: Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free [NBP3-25466] -

Western Blot: Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free [NBP3-25466] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with FGL1 antibody [HL2317] (NBP3-25466) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free [NBP3-25466] -

Immunocytochemistry/Immunofluorescence: Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free [NBP3-25466] - FGL1 antibody [HL2317] detects FGL1 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: FGL1 stained by FGL1 antibody [HL2317] (NBP3-25466) diluted at 1:250. Blue: Fluoroshield with DAPI .

Applications for Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Hepassocin/FGL1

Hepassocin, also known as fibrinogen like 1 (FGL1) and hepatocyte-derived fibrinogen-related protein 1 (HFREP1), is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. Hepassocin/FGL1 may play a role in the development of hepatocellular carcinomas. Hepassocin/FGL1 is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin/FGL1 is upregulated during liver regeneration following partial hepatectomy.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Gene Symbol

FGL1

Additional Hepassocin/FGL1 Products

Product Documents for Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hepassocin/FGL1 Antibody (HL2317) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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