Hepcidin Antimicrobial Peptide Antibody (4C5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26242
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4C5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from Human Hepcidin Antimicrobial Peptide [UniProt P81172]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hepcidin Antimicrobial Peptide Antibody (4C5)
Immunohistochemistry: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] -
Immunohistochemistry: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] - Image of Hepcidin Antimicrobial Peptide Antibody (4C5) diluted at 1:215 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] -
Western Blot: Hepcidin Antimicrobial Peptide Antibody (4C5) [NBP3-26242] - Positive Western Blot detected in: Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate.All lanes: Hepcidin Antimicrobial Peptide Antibody at 2.15ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 10 KDa
Observed band size: 25 KDa
Applications for Hepcidin Antimicrobial Peptide Antibody (4C5)
Application
Recommended Usage
Immunohistochemistry
1:50-1:200
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Hepcidin
Long Name
Hepcidin Antimicrobial Peptide
Alternate Names
HAMP, HEPC, HFE2B, LEAP1, PLTR
Gene Symbol
HAMP
Additional Hepcidin Products
Product Documents for Hepcidin Antimicrobial Peptide Antibody (4C5)
Product Specific Notices for Hepcidin Antimicrobial Peptide Antibody (4C5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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