Hepcidin Antimicrobial Peptide Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36960

Novus Biologicals, part of Bio-Techne
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NBP1-36960
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Hepcidin Antibodies »

Product Specifications

Immunogen

Peptide with sequence TSGSVFPQQTGQLAE-C corresponding to N-Terminus (near) according to NP_066998.1.

Epitope

N Terminus (near)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Hepcidin Antimicrobial Peptide Antibody

Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody [NBP1-36960]

Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody [NBP1-36960]

Immunohistochemistry-Paraffin: Hepcidin Antimicrobial Peptide Antibody [NBP1-36960] - (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.

Applications for Hepcidin Antimicrobial Peptide Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Immunohistochemistry-Paraffin

3.75 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

1 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 28kDa band in Human Liver lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.41kDa according to NP_066998.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Heart.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hepcidin

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure.

Long Name

Hepcidin Antimicrobial Peptide

Alternate Names

HAMP, HEPC, HFE2B, LEAP1, PLTR

Entrez Gene IDs

57817 (Human)

Gene Symbol

HAMP

UniProt

NP_066998.1 (Human)

Additional Hepcidin Products

Product Documents for Hepcidin Antimicrobial Peptide Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hepcidin Antimicrobial Peptide Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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