Hephaestin Antibody (2D3)
Novus Biologicals, part of Bio-Techne | Catalog # H00009843-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 2D3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
HEPH (NP_620074, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TRGHHTDVANIFPATFVTAEMVPWEPGTWLISCQVNSHFRDGMQALYKVKSCSMAPPVDLLTGKVRQYFIEAHEIQWDYGPMGHDGSTGKNLREPGSISDKFFQKSSSRI
Reactivity Notes
Human. Other species not tested.
Specificity
HEPH (2D3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Hephaestin Antibody (2D3)
![Western Blot: Hephaestin Antibody (2D3) [H00009843-M01]](https://resources.bio-techne.com/images/products/Hephaestin-Antibody-2D3-Western-Blot-H00009843-M01-img0001.jpg "Western Blot: Hephaestin Antibody (2D3) [H00009843-M01]")
Western Blot: Hephaestin Antibody (2D3) [H00009843-M01]
Western Blot: Hephaestin Antibody (2D3) [H00009843-M01] - PH monoclonal antibody (M01), clone 2D3 Western Blot analysis of HEPH expression in K-562 ( Cat # L009V1 ).
Sandwich ELISA: Hephaestin Antibody (2D3) [H00009843-M01] - Detection limit for recombinant GST tagged HEPH is approximately 0.3ng/ml as a capture antibody.
Applications for Hephaestin Antibody (2D3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Hephaestin
Alternate Names
EC 1.16.3.1, hephaestin, KIAA0698CPL
Entrez Gene IDs
9843 (Human)
Gene Symbol
HEPH
OMIM
300167 (Human)
UniProt
Additional Hephaestin Products
Product Documents for Hephaestin Antibody (2D3)
Product Specific Notices for Hephaestin Antibody (2D3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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