Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HERC2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 4784 and 4834 of human hect domain and RLD 2 using the numbering given in entry NP_004658.3 (GeneID 8924).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

527 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HERC2 Antibody

Western Blot: HERC2 Antibody [NBP1-05970]

Western Blot: HERC2 Antibody [NBP1-05970]

Western Blot: HERC2 Antibody [NBP1-05970] - Detection of Mouse HERC2- Whole cell lysate (50 ug) from NIH3T3, TCMK-1, Renca, 4T1, and CT26.WT cells. Affinity purified rabbit anti-HERC2 antibody used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: HERC2 Antibody [NBP1-05970]

Immunoprecipitation: HERC2 Antibody [NBP1-05970]

Immunoprecipitation: HERC2 Antibody [NBP1-05970] - Detection of human HERC2 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HERC2 antibody NBP1-05970 (lot NBP1-05970-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HERC2 was also immunoprecipitated by a previous lot (lot NBP1-05970-1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Applications for HERC2 Antibody

Application
Recommended Usage

Immunoprecipitation

2- 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HERC2

HERC2 (HECT domain and RCC1-like domain-containing protein 2) belongs to the HERC family of ubiquitin ligases. A single nucleotide polymorphism (SNP) in an evolutionarily conserved region of the HERC2 gene is associated with blue eye color. Additionally, it has been reported that gene-gene interactions between HERC2, OCA2, and MC1R may influence eye, skin, and hair color.

Long Name

E3 ubiquitin-protein ligase HERC2

Alternate Names

EC 2.3.2.26

Entrez Gene IDs

8924 (Human)

Gene Symbol

HERC2

UniProt

O95714 (Human)

Additional HERC2 Products

Product Documents for HERC2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HERC2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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