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HERC3 Antibody (2C1)

Novus Biologicals, part of Bio-Techne | Catalog # H00008916-M01

Novus Biologicals, part of Bio-Techne
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H00008916-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HERC3 (NP_055421, 1 a.a. ~ 109 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLCWGYWSLGQPGISTNLQGIVAEPQVCGFISDRSVKEVACGGNHSVFLLEDGEVYTCGLNTKGQLGHEREGNKPEQIGALADQHIIHVACGESHSLALSDRGQLFSWG

Localization

Cytoplasmic. Cytoplasmic vesicle. Note=Also found in vesicular-like structures.

Specificity

HERC3 - hect domain and RLD 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HERC3 Antibody (2C1)

Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01]

Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01]

Immunohistochemistry-Paraffin: HERC3 Antibody (2C1) [H00008916-M01] - Analysis of monoclonal antibody to HERC3 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
Sandwich ELISA: HERC3 Antibody (2C1) [H00008916-M01] - Detection limit for recombinant GST tagged HERC3 is approximately 0.1ng/ml as a capture antibody.

Applications for HERC3 Antibody (2C1)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HERC3

HERC3 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM]

Alternate Names

EC 6.3.2, HECT domain and RCC1-like domain-containing protein 3, hect domain and RLD 3, KIAA0032EC 6.3.2.-, probable E3 ubiquitin-protein ligase HERC3

Entrez Gene IDs

8916 (Human)

Gene Symbol

HERC3

OMIM

605200 (Human)

Additional HERC3 Products

Product Documents for HERC3 Antibody (2C1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HERC3 Antibody (2C1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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