HEXDC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83574

Novus Biologicals, part of Bio-Techne
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NBP1-83574
NBP1-83574-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HEXDC Antibodies »

Product Summary for HEXDC Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASAFKGATGPSQAVPPVEHHLRNHVQWLQVAGSGPTDSLQGIILTGWQRYDHYSVLCELLPAGVPSLAACLQLLLRGGFDEDVKAKVEN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HEXDC Antibody

Western Blot: HEXDC Antibody [NBP1-83574]

Western Blot: HEXDC Antibody [NBP1-83574]

Western Blot: HEXDC Antibody [NBP1-83574] - Analysis in control (vector only transfected HEK293T lysate) and HEXDC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: HEXDC Antibody [NBP1-83574]

Immunocytochemistry/ Immunofluorescence: HEXDC Antibody [NBP1-83574]

Immunocytochemistry/Immunofluorescence: HEXDC Antibody [NBP1-83574] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center & mitochondria.
Immunohistochemistry-Paraffin: HEXDC Antibody [NBP1-83574]

Immunohistochemistry-Paraffin: HEXDC Antibody [NBP1-83574]

Immunohistochemistry-Paraffin: HEXDC Antibody [NBP1-83574] - Staining of human cerebellum shows strong cytoplasmic positivity in granular layer cells.

Applications for HEXDC Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HEXDC

HEXDC has hexosaminidase activity

Alternate Names

Beta-hexosaminidase D, Beta-N-acetylhexosaminidase, EC 3.2.1.52, FLJ23825, hexosaminidase (glycosyl hydrolase family 20, catalytic domain) containing, hexosaminidase D, Hexosaminidase domain-containing protein, N-acetyl-beta-galactosaminidase

Gene Symbol

HEXDC

Additional HEXDC Products

Product Documents for HEXDC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HEXDC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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