HFE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00003077-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HFE Antibodies »

Product Specifications

Immunogen

HFE (NP_000401.1, 1 a.a. - 348 a.a.) full-length human protein. MGPRARPALLLLMLLQTAVLQGRLLRSHSLHYLFMGASEQDLGLSLFEALGYVDDQLFVFYDHESRRVEPRTPWVSSRISSQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSKESHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQVPPLVKVTHHVTSSVTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEHPGLDQPLIVIWEPSPSGTLVIGVISGIAVFVVILFIGILFIILRKRQGSRGAMGHYVLAERE

Specificity

HFE - hemochromatosis,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for HFE Antibody

Western Blot: HFE Antibody [H00003077-B01P]

Western Blot: HFE Antibody [H00003077-B01P]

Western Blot: HFE Antibody [H00003077-B01P] - Analysis of HFE expression in HepG2.
Flow Cytometry: HFE Antibody [H00003077-B01P]

Flow Cytometry: HFE Antibody [H00003077-B01P]

Flow Cytometry: HFE Antibody [H00003077-B01P] - Analysis of negative control 293 cells (Black) and HFE expressing 293 cells (Green) using HFE purified mouse polyclonal antibody.
Western Blot: HFE Antibody [H00003077-B01P]

Western Blot: HFE Antibody [H00003077-B01P]

Western Blot: HFE Antibody [H00003077-B01P] - Analysis of HFE expression in transfected 293T cell line by HFE polyclonal antibody. Lane 1: HFE transfected lysate(38.28 KDa). Lane 2: Non-transfected lysate.

Applications for HFE Antibody

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It has been used for FACS.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HFE

The protein encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined. [provided by RefSeq]

Long Name

Homeostatic Iron Regulator

Alternate Names

HFE1, HLA-H, MVCD7, TFQTL2

Entrez Gene IDs

3077 (Human)

Gene Symbol

HFE

UniProt

NP_000401.1 (Human)

Additional HFE Products

Product Documents for HFE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HFE Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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