HFE Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83250

Novus Biologicals, part of Bio-Techne
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NBP1-83250
NBP1-83250-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HFE Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YLERDCPAQLQQLLELGRGVLDQQVPPLVKVTHHVTSSVTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEHPGLDQPLIVIWEPSPS

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23991213)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HFE Antibody

Western Blot: HFE Antibody [NBP1-83250]

Western Blot: HFE Antibody [NBP1-83250]

Western Blot: HFE Antibody [NBP1-83250] - Analysis in control (vector only transfected HEK293T lysate) and HFE over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250]

Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250]

Immunohistochemistry-Paraffin: HFE Antibody [NBP1-83250] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.
Immunohistochemistry: HFE Antibody [NBP1-83250]

Immunohistochemistry: HFE Antibody [NBP1-83250]

Immunohistochemistry: HFE Antibody [NBP1-83250] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
View 1 more images

Applications for HFE Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HFE

HFE is encoded by this gene is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin (beta2M). It is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in this gene. At least nine alternatively spliced variants have been described for this gene. Additional variants have been found but their full-length nature has not been determined.

Long Name

Homeostatic Iron Regulator

Alternate Names

HFE1, HLA-H, MVCD7, TFQTL2

Gene Symbol

HFE

Additional HFE Products

Product Documents for HFE Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HFE Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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