Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12.1

Format

BSA Free

Concentration

1 mg/ml

View all HGFR/c-MET Antibodies »

Product Specifications

Immunogen

Bacterially expressed human HGFR/c-MET alpha chain

Specificity

Clone 12.1 recognizes a denatured epitope. The amino acid sequence of this epitope is LEHPDCFPCQDCSSK.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This clone is also referred to as 5E7/G7.

Scientific Data Images for HGFR/c-MET Antibody (12.1) - BSA Free

Western Blot: HGFR/c-MET Antibody (12.1)BSA Free [NBP2-50169]

Western Blot: HGFR/c-MET Antibody (12.1)BSA Free [NBP2-50169]

Western Blot: HGF R/c-MET Antibody (12.1) [NBP2-50169] - Endogenous c-Met expression in a panel of human gastric cell lines. mAb12.1-HRP recognises 190kD pro-s-Met and 50kD mature c-Met alpha chain. mAb 12.1-HRP does not recognise the 145kD mature c-Met beta-chain.

Applications for HGFR/c-MET Antibody (12.1) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
Positive control(s): SNU-5, U-87MG and MKN45 cells (negative control: T47D cells).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HGFR/c-MET

Met is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (1). Hereditary papillary renal carcinoma (HPRC) is a recently recognized form of inherited kidney cancer Results suggest that missense mutations located in the MET proto-oncogene lead to constitutive activation of the Met protein and papillary renal carcinomas (3)

Long Name

Hepatocyte Growth Factor Receptor

Alternate Names

c-MET, cMET, HGF R, MET

Gene Symbol

MET

Additional HGFR/c-MET Products

Product Documents for HGFR/c-MET Antibody (12.1) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HGFR/c-MET Antibody (12.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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