HGFR/c-MET Antibody (556)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43641

Novus Biologicals, part of Bio-Techne
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NBP2-43641
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 556

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HGFR/c-MET Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HGFR/c-MET. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

156 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HGFR/c-MET Antibody (556)

Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641]

Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641]

Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [556]. HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641]

Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641]

Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556].
Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641]

Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641]

Immunohistochemistry-Paraffin: HGFR/c-MET Antibody (556) [NBP2-43641] - Human esophageal carcinoma. c-Met antibody [556] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
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Applications for HGFR/c-MET Antibody (556)

Application
Recommended Usage

Flow Cytometry

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HGFR/c-MET

Met is a receptor protein-tyrosine kinase (RPTK) for hepatocyte growth factor (HGF), which is a multifunctional cytokine controlling cell growth, morphogenesis, and motility. Met overexpression has been identified in a variety of human cancers (1). Met kinase domain possesses unique features that distinguish met from other members of the src family of protein tyrosine kinases. These results also demonstrate that the product of the activated met gene is a fusion protein and that the amino terminal end of this fusion protein exhibits homology to laminin B1 (2). Data suggest that RanBP9, functioning as an adaptor protein for the Met tyrosine kinase domain, can augment the HGF-Met signaling pathway and that RanBP9 overexpression may cause constitutive activation of the Ras signaling pathway (1). Hereditary papillary renal carcinoma (HPRC) is a recently recognized form of inherited kidney cancer Results suggest that missense mutations located in the MET proto-oncogene lead to constitutive activation of the Met protein and papillary renal carcinomas (3)

Long Name

Hepatocyte Growth Factor Receptor

Alternate Names

c-MET, cMET, HGF R, MET

Gene Symbol

MET

Additional HGFR/c-MET Products

Product Documents for HGFR/c-MET Antibody (556)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HGFR/c-MET Antibody (556)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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