Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 850 and 900 of human MAPK/ERK kinase kinase kinase 4 (hepatocyte progenitor kinase-like/germinal center kinase-like kinase) using the numbering given in entry AAO32626.1 (GeneID 94

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HGK/MAP4K4 Antibody

Western Blot: HGK/MAP4K4 Antibody [NB100-74630]

Western Blot: HGK/MAP4K4 Antibody [NB100-74630]

Western Blot: HGK/MAP4K4 Antibody [NB100-74630] - Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAP4K4/HGK antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: HGK/MAP4K4 Antibody [NB100-74630]

Immunoprecipitation: HGK/MAP4K4 Antibody [NB100-74630]

Immunoprecipitation: HGK/MAP4K4 Antibody [NB100-74630] - Detection of human MAP4K4/HGK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAP4K4/HGK antibody NB100-74630 (lot 2) used for IP at 6 ug per reaction. MAP4K4/HGK was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-MAP4K4/HGK antibody NB100-74629. For blotting immunoprecipitated MAP4K4/HGK, NB100-74630 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for HGK/MAP4K4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HGK

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase

Alternate Names

MAP4K4, MEKKK4

Entrez Gene IDs

9448 (Human)

Gene Symbol

MAP4K4

UniProt

AAO32626.1 (Human)

Additional HGK Products

Product Documents for HGK/MAP4K4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HGK/MAP4K4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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