HGK/MAP4K4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84663
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Predicted:
Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: AEPQVQWSHLASLKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRVPVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSI
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HGK/MAP4K4 Antibody
![Western Blot: HGK/MAP4K4 Antibody [NBP1-84663]](https://resources.bio-techne.com/images/products/HGK-MAP4K4-Antibody-Western-Blot-NBP1-84663-img0005.jpg "Western Blot: HGK/MAP4K4 Antibody [NBP1-84663]")
Western Blot: HGK/MAP4K4 Antibody [NBP1-84663]
Western Blot: HGK/MAP4K4 Antibody [NBP1-84663] - Analysis in control (vector only transfected HEK293T lysate) and MAP4K4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663]](https://resources.bio-techne.com/images/products/HGK-MAP4K4-Antibody-Immunohistochemistry-Paraffin-NBP1-84663-img0004.jpg "Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663]")
Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663]
Immunohistochemistry-Paraffin: HGK/MAP4K4 Antibody [NBP1-84663] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemistry: HGK/MAP4K4 Antibody [NBP1-84663] -
Immunohistochemistry of candidate biomarkers in prostate cancer. Representative immunohistochemical staining of ACPP, ADAM9, ALDH1A2, CASR, CCND1, CCPG1, CD34, CD44, CD44v6, CHGA, CHMP1A, EI24, ENO2, GADD45B, HA, HAS2, HES6, HMMR, HOXC6, HYAL1, IGF1, IQCK, MAP4K4, MKI67, PAGE4, PLIN2, PTEN, SIAH2, SMAD4, SOX9, SPP1, SYP, & TP53 from prostate cancer tissue microarrays. Scale bar represents 50 μm. Image collected & cropped by CiteAb from the following publication (https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-14-244), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for HGK/MAP4K4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HGK
Long Name
Hepatocyte Pogenitor Kinase-like/Germinal Center Knase-like Kinase
Alternate Names
MAP4K4, MEKKK4
Gene Symbol
MAP4K4
Additional HGK Products
Product Documents for HGK/MAP4K4 Antibody
Product Specific Notices for HGK/MAP4K4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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