HIF-1 alpha Antibody (HA111) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80763
![Western Blot: HIF-1 alpha Antibody (HA111)Azide and BSA Free [NBP2-80763]](https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-HA111-Azide-and-BSA-Free-Western-Blot-NBP2-80763-img0001.jpg "Western Blot: HIF-1 alpha Antibody (HA111)Azide and BSA Free [NBP2-80763]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2 Alpha Clone # HA111
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for HIF-1 alpha Antibody (HA111) - Azide and BSA Free
Immunogen
This HIF-1 alpha Antibody (HA111) was developed against Human HIF-1 alpha, corresponding to amino acids 329 - 530 [Uniprot# Q16665].
Reactivity Notes
Mouse reactivity reported in literature (PMID: 16738327). Rabbit reactivity reported in scientific literature (PMID: 16738327).
Localization
Cytoplasmic in normoxia, nuclear translocation in response to hypoxia.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2 Alpha
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HIF-1 alpha Antibody (HA111) - Azide and BSA Free
Western Blot: HIF-1 alpha Antibody (HA111)Azide and BSA Free [NBP2-80763]
Western Blot: HIF-1 alpha Antibody (HA111) - Azide and BSA Free [NBP2-80763] - Detection of HIF-1 alpha (125-130 kDa) from human placental villous explant total protein. Image from the standard format of this antibody.![Immunohistochemistry: HIF-1 alpha Antibody (HA111) - Azide and BSA Free [NBP2-80763]](https://resources.bio-techne.com/images/products/HIF-1-alpha-Antibody-HA111-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80763-img0002.jpg "Immunohistochemistry: HIF-1 alpha Antibody (HA111) - Azide and BSA Free [NBP2-80763]")
Immunohistochemistry: HIF-1 alpha Antibody (HA111) - Azide and BSA Free [NBP2-80763]
Immunohistochemistry: HIF-1 alpha Antibody (HA111) - Azide and BSA Free [NBP2-80763] - HIF-1 antibody was tested in human ovarian cancer tumor xenograft using DAB with hematoxylin counterstain. Image from the standard format of this antibody.Applications for HIF-1 alpha Antibody (HA111) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:100
Immunoprecipitation
1:10 - 1:500. Use reported in scientific literature (PMID 18222538)
Western Blot
0.5 - 1.0 mg/ml
Application Notes
In IHC-P, staining was observed in the nucleus of human ovarian cancer tumor. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Nuclear extracts should be used for Western blot analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HIF-1 alpha/HIF1A
HIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. In its active form under hypoxic conditions, HIF-1 is stabilized by the formation of a heterodimer of HIF-1 alpha and ARNT/HIF-1 beta subunits. Nuclear HIF-1 engages p300/CBP for binding to hypoxic response elements (HREs). This process induces transcription and regulation of genes including EPO, VEGF, iNOS2, ANGPT1 and OCT4 (4,5).
Under normoxic conditions, the HIF-1 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system. This process is mediated by prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-402 and Pro-564) within the oxygen-dependent degradation domain of HIF-1 alpha. Once hydroxylated, HIF-1 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (4). pVHL dependent regulation of HIF-1 alpha plays a role in normal physiology and disease states. Regulation of HIF-1 alpha by pVHL is critical for the suppressive function of FoxP3+ regulatory Tcells (6). Repression of pVHL expression in chronic lymphocytic leukemia (CLL) B cells leads to HIF-1 alpha stabilization and increased VEGF secretion (7).
References
1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.
2. Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413
3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y
4. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483
5. Dengler, V. L., Galbraith, M. D., & Espinosa, J. M. (2014). Transcriptional regulation by hypoxia inducible factors. Critical Reviews in Biochemistry and Molecular Biology. https://doi.org/10.3109/10409238.2013.838205
6. Lee, J. H., Elly, C., Park, Y., & Liu, Y. C. (2015). E3Ubiquitin Ligase VHL Regulates Hypoxia-Inducible Factor-1 alpha to Maintain Regulatory T Cell Stability and Suppressive Capacity. Immunity. https://doi.org/10.1016/j.immuni.2015.05.016
7. Ghosh, A. K., Shanafelt, T. D., Cimmino, A., Taccioli, C., Volinia, S., Liu, C. G., ... Kay, N. E. (2009). Aberrant regulation of pVHL levels by microRNA promotes the HIF/VEGF axis in CLL B cells. Blood. https://doi.org/10.1182/blood-2008-10-185686
Long Name
Hypoxia Inducible Factor 1 Subunit Alpha
Alternate Names
BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8
Gene Symbol
HIF1A
Additional HIF-1 alpha/HIF1A Products
Product Documents for HIF-1 alpha Antibody (HA111) - Azide and BSA Free
Product Specific Notices for HIF-1 alpha Antibody (HA111) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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