HIF-3 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00064344-B02P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HIF-3 alpha/HIF3A Antibodies »

Product Specifications

Immunogen

HIF3A (AAH80551.1, 1 a.a. - 669 a.a.) full-length human protein. MALGLQRARSTTELRKEKSRDAARSRRSQETEVLYQLAHTLPFARGVSAHLDKASIMRLTISYLRMHRLCAAGEWNQVGAGGEPLDACYLKALEGFVMVLTAEGDMAYLSENVSKHLGLSQLELIGHSIFDFIHPCDQEELQDALTPQQTLSRRKVEAPTERCFSLRMKSTLTSRGRTLNLKAATWKVLNCSGHMRAYKPPAQTSPAGSPDSEPPLQCLVLICEAIPHPGSLEPPLGRGAFLSRHSLDMKFTYCDDRIAEVAGYSPDDLIGCSAYEYIHALDSDAVSKSIHTLLSKGQAVTGQYRFLARSGGYLWTQTQATVVSGGRGPQSESIVCVHFLISRVEETGVVLSLEQTEQHSRRPIQRGAPSQKDTPNPGDSLDTPGPRILAFLHPPSLSEAALAADPRRFCSPDLRRLLGPILDGASVAATPSTPLATRHPQSPLSADLPDELPVGTENVHRLFTSGKDTEAVETDLDIAQDADALDLEMLAPYISMDDDFQLNASEQLPRAYHRPLGAVPRPRARSFHGLSPPALEPSLLPRWGSDPRLSCSSPSRGDPSASSPMAGARKRTLAQSSEDEDEGVELLGVRPPKRSPSPEHENFLLFPLSLSFLLTGGPAPGSLQDPSTPLLNLNEPLGLGPSLLSPYSDEDTTQPGGPFQPRAGSAQAD

Specificity

HIF3A - hypoxia inducible factor 3, alpha subunit,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for HIF-3 alpha Antibody

Western Blot: HIF-3 alpha Antibody [H00064344-B02P]

Western Blot: HIF-3 alpha Antibody [H00064344-B02P]

Western Blot: HIF-3 alpha Antibody [H00064344-B02P] - Analysis of HIF3A expression in transfected 293T cell line by HIF3A polyclonal antibody. Lane1:HIF3A transfected lysate(73.59 KDa). Lane2:Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HIF-3 alpha Antibody [H00064344-B02P]

Immunocytochemistry/ Immunofluorescence: HIF-3 alpha Antibody [H00064344-B02P]

Immunocytochemistry/Immunofluorescence: HIF-3 alpha Antibody [H00064344-B02P] - Analysis of purified antibody to HIF3A on HeLa cell. (antibody concentration 10 ug/ml)

Applications for HIF-3 alpha Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HIF-3 alpha/HIF3A

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. At least three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Hypoxia Inducible Factor 3 Subunit Alpha

Alternate Names

BHLHe17, HIF3A, IPAS, MOP7, PASD7

Entrez Gene IDs

64344 (Human)

Gene Symbol

HIF3A

UniProt

AAH80551.1 (Human)

Additional HIF-3 alpha/HIF3A Products

Product Documents for HIF-3 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HIF-3 alpha Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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