HINT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81981

Novus Biologicals, part of Bio-Techne
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NBP1-81981
NBP1-81981-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HINT3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAEEQVNRSAGLAPDCEASATAETTVSSVGTCEAAAKSPEPKDYDSTCVFCRIAGRQDPGTELLHCENEDLICFKDIKPAATHHYLVVP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HINT3 Antibody

Western Blot: HINT3 Antibody [NBP1-81981]

Western Blot: HINT3 Antibody [NBP1-81981]

Western Blot: HINT3 Antibody [NBP1-81981] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4.
Immunocytochemistry/ Immunofluorescence: HINT3 Antibody [NBP1-81981]

Immunocytochemistry/ Immunofluorescence: HINT3 Antibody [NBP1-81981]

Immunocytochemistry/Immunofluorescence: HINT3 Antibody [NBP1-81981] - Staining of human cell line A-431 shows positivity in nucleoli, cytoplasm & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HINT3 Antibody [NBP1-81981]

Immunohistochemistry-Paraffin: HINT3 Antibody [NBP1-81981]

Immunohistochemistry-Paraffin: HINT3 Antibody [NBP1-81981] - Staining of human tonsil shows no positivity in germinal center cells as expected.
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Applications for HINT3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HINT3

Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM]

Alternate Names

EC 3.-, FLJ33126, FLJ99898, HINT-3, HINT4, histidine triad nucleotide binding protein 3, histidine triad nucleotide-binding protein 3, HIT-like protein, MGC22976

Entrez Gene IDs

135114 (Human)

Gene Symbol

HINT3

UniProt

Q9NQE9 (Human)

Additional HINT3 Products

Product Documents for HINT3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HINT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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