HINT3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97827

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HINT3 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human HINT3 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for HINT3 Antibody

Western Blot: HINT3 Antibody [NBP2-97827]

Western Blot: HINT3 Antibody [NBP2-97827]

Western Blot: HINT3 Antibody [NBP2-97827] - Anti-HINT3 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 20 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: HINT3 Antibody [NBP2-97827]

Immunocytochemistry/ Immunofluorescence: HINT3 Antibody [NBP2-97827]

Immunocytochemistry/Immunofluorescence: HINT3 Antibody [NBP2-97827] - Immunofluorescence staining of HINT3 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HINT3 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.
Immunohistochemistry-Paraffin: HINT3 Antibody [NBP2-97827]

Immunohistochemistry-Paraffin: HINT3 Antibody [NBP2-97827]

Immunohistochemistry-Paraffin: HINT3 Antibody [NBP2-97827] - Immunochemical staining of human HINT3 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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Applications for HINT3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HINT3

Histidine triad proteins, such as HINT3, are nucleotide hydrolases and transferases that act on the alpha-phosphate of ribonucleotides (Brenner, 2002 [PubMed 12119013]).[supplied by OMIM]

Alternate Names

EC 3.-, FLJ33126, FLJ99898, HINT-3, HINT4, histidine triad nucleotide binding protein 3, histidine triad nucleotide-binding protein 3, HIT-like protein, MGC22976

Gene Symbol

HINT3

Additional HINT3 Products

Product Documents for HINT3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HINT3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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