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HIP-55 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31026

Novus Biologicals, part of Bio-Techne
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NBP1-31026

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HIP-55. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (84%).

Localization

Cytoplasm, cytoskeleton, Cell projection, lamellipodium

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HIP-55 Antibody

Western Blot: HIP-55 Antibody [NBP1-31026]

Western Blot: HIP-55 Antibody [NBP1-31026]

Western Blot: HIP-55 Antibody [NBP1-31026] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:MOLT4 10% SDS PAGE, antibody diluted at 1:1500.
Immunocytochemistry/ Immunofluorescence: HIP-55 Antibody [NBP1-31026]

Immunocytochemistry/ Immunofluorescence: HIP-55 Antibody [NBP1-31026]

Immunocytochemistry/Immunofluorescence: HIP-55 Antibody [NBP1-31026] - Sample: HeLa cells were fixed in iced-cold MeOH for 5 min. Green: DBNL protein stained by HIP55 antibody, N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-31026]

Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-31026]

Immunohistochemistry-Paraffin: HIP-55 Antibody [NBP1-31026] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.

Applications for HIP-55 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HIP-55

HIP55 possesses a SCAD domain that binds to polymerized actin and an SH3 domain. The latter is suggested to bind to Dynamin 1, a protein involved in the mechanism responsible for the formation of endocytotic vesicles.

Alternate Names

ABP1, Cervical SH3P7, CMAP, drebrin-F, drebrin-like, drebrin-like protein, HIP-55SH3P7Cervical mucin-associated protein, HPK1-interacting protein of 55 kDa, SH3 domain-containing protein 7, src homology 3 domain-containing protein HIP-55

Gene Symbol

DBNL

Additional HIP-55 Products

Product Documents for HIP-55 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HIP-55 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.