Hip Antibody (HL1737) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25505
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (98%), Rat (98%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1737
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Hip. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (82%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hip Antibody (HL1737) - Azide and BSA Free
Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] -
Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] -
Western Blot: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HHIP antibody [HL1737] (NBP3-25505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] -
Immunohistochemistry-Paraffin: Hip Antibody (HL1737) - Azide and BSA Free [NBP3-25505] - HHIP antibody [HL1737] detects HHIP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. HHIP stained by HHIP antibody [HL1737] (NBP3-25505) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Hip Antibody (HL1737) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Hip
Long Name
Hedgehog-interacting Protein
Alternate Names
HHIP
Gene Symbol
HHIP
Additional Hip Products
Product Documents for Hip Antibody (HL1737) - Azide and BSA Free
Product Specific Notices for Hip Antibody (HL1737) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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