His Tag Antibody (3D5) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52637
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Epitope Tag
Applications
ELISA, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 3D5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
This His Tag Antibody (3D5) - Chimeric was developed against purified his tagged Fab fragment.
Specificity
This antibody binds to carboxy-terminal oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity for internal His-tags but no affinity for N-terminal His-tags.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free
Western Blot: His Tag Antibody (3D5)ChimericAzide and BSA Free [NBP2-52637]
Western Blot: His Tag Antibody (3D5) [NBP2-52637] - Staining of Multitag protein at 25 ng (A), 10 ng (B) and 5 ng (C) amounts. Used at a dilutino of 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.Simple Western: His Tag Antibody (3D5)ChimericAzide and BSA Free [NBP2-52637]
Simple Western: His Tag Antibody (3D5) - Chimeric [NBP2-52637] - His Tag Antibody (3D5) [NBP2-52637] - Human purified protein. Simple Western image submitted by a verified customer review.Applications for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Western Blot
1:100 - 1:2000
Application Notes
This is a chimeric rabbit antibody, based on the original mouse IgG2b format, created for improved compatibility with existing reagents, assays and techniques.
Reviewed Applications
Read 1 review rated 5 using NBP2-52637 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: His Tag
References
1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0
2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6
3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free
Product Specific Notices for His Tag Antibody (3D5) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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