His Tag PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC0501P

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Multi-Species

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # AD1.1.10R

View all His Tag Antibodies »

Product Specifications

Immunogen

His-tagged peptide

Specificity

Detects proteins containing accessible consecutive histidine regions.  The antibody detects His tags located at the amino- or carboxyl- terminus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for His Tag PE-conjugated Antibody

Detection of protein binding with Mouse anti-His Tag PE in HEK293 cells by Flow Cytometry

In a functional flow cytometry test, recombinant SARS-CoV2 S1 RBD His-tagged protein (50 ng/mL, 10500-CV) binds to HEK293 cells transfected with Human ACE-2 and eGFP. (A) Protein binding was detected with Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) or Mouse Anti-His Tag PE conjugated Monoclonal Antibody (Catalog # IC0501P) alone. View our protocol for View our protocol for Staining Membrane-associated Proteins.

Applications for His Tag PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 cells transfected with Human ACE-2 and eGFP.  

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: His Tag

Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected or purified by using anti-polyHis antibodies.

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for His Tag PE-conjugated Antibody

For research use only

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