Histone Deacetylase 2/HDAC2 Antibody (10R7O1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15824

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15824-100ul
NBP3-15824-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10R7O1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Histone Deacetylase 2/HDAC2 Antibodies »

Product Summary for Histone Deacetylase 2/HDAC2 Antibody (10R7O1)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 2/HDAC2 (NP_001518.3). MAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYSKQMQRFNVGED

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone Deacetylase 2/HDAC2 Antibody (10R7O1)

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824]

Western Blot: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Western blot analysis of extracts of various cell lines, using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824]

Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824]

Immunocytochemistry/Immunofluorescence: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunofluorescence analysis of NIH-3T3 cells using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824]

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824]

Immunohistochemistry-Paraffin: Histone Deacetylase 2/HDAC2 Antibody (10R7O1) [NBP3-15824] - Immunohistochemistry of paraffin-embedded mouse brain using Histone Deacetylase 2/HDAC2 antibody (NBP3-15824) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Histone Deacetylase 2/HDAC2 Antibody (10R7O1)

Application
Recommended Usage

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 2/HDAC2

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human.

Long Name

Histone Deacetylase 2

Alternate Names

HDAC2, YAF1

Gene Symbol

HDAC2

Additional Histone Deacetylase 2/HDAC2 Products

Product Documents for Histone Deacetylase 2/HDAC2 Antibody (10R7O1)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Histone Deacetylase 2/HDAC2 Antibody (10R7O1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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